Ilana Chromogenic wa laarin awọn ilana mẹta ti o tun ni ilana gel-clot ati ilana turbidimetric lati wa tabi ṣe iṣiro awọn endotoxins lati awọn kokoro arun Gram-negative nipa lilo amoebocyte lysate ti a fa jade lati inu ẹjẹ buluu ti akan horseshoe (Limulus polyphemus tabi Tachypleus tridentatus).O le jẹ tito lẹtọ bi igbeyẹwo chromogenic-ipari tabi igbeyẹwo kinetic-chromogenic ti o da lori ilana igbelewọn pato ti o ṣiṣẹ.
Ilana ifaseyin ni pe: amebocyte lysate ni kasikedi ti awọn enzymu protease serine (proenzymes) eyiti o le muu ṣiṣẹ nipasẹ awọn endotoxins kokoro-arun.Endotoxins mu awọn proenzymes ṣiṣẹ lati ṣe awọn enzymu ti a mu ṣiṣẹ (ti a npe ni coagulase), igbehin n ṣe itọpa pipin ti sobusitireti ti ko ni awọ, ti o tu ọja awọ ofeefee kan silẹ pNA.pNA ti a tu silẹ le jẹ iwọn photometrically ni 405nm.Ati ifasilẹ naa ni ibamu pẹlu ifọkansi endotoxin, lẹhinna ifọkansi endotoxin le ṣe iwọn ni ibamu.
Akoko ifiweranṣẹ: Oṣu Kẹsan-29-2019
