Itọsọnafun Ipari Bioendo-point Chromogenic LAL Awọn ohun elo Idanwo:
TAL reagent, ie lyophilized amebocyte lysate ti a fa jade lati inu ẹjẹ buluu ti horseshore akan (Limulus polyphemus tabi Tachypleus tridentatus), nigbagbogbo ni iṣẹ lati ṣe idanwo endotoxins kokoro-arun.
Ni Bioendo, a ṣe awọn ohun elo lati ṣe ipari-point chromogenic TAL assay / LAL assay, eyiti o pẹlu gbogbo awọn reagents pataki fun BET nipasẹ iyipada ninu idagbasoke awọ, ati gba alabara laaye lati gba awọn abajade deede ni igba diẹ.
Awọn ilana ti wiwa chromogenic aaye ipari pẹlu reagent TAL ni pe: ifa enzymatic ṣẹlẹ nigbati awọn endotoxins kokoro ba mu ifosiwewe C ṣiṣẹ, ati pe coagulase ti ṣẹda ni ibamu.Lẹhinna coagulase pin sobusitireti ti ko ni awọ si idasilẹ pNA ọja awọ ofeefee kan.pNA ti o tu silẹ le jẹ iwọn photometrically ni 405nm.Niwọn igba ti ifasilẹ naa ti ni ibatan daadaa pẹlu ifọkansi endotoxin, ifọkansi endotoxin le ṣe iwọn ni ibamu.
EC64405, ie BioendoEC Apo Idanwo Endotoxin (Ipari-point Chromogenic Assay), jẹ aaye ipari Chromogenic TAL assay / ohun elo idanwo LAL ti o le lo lati ṣe wiwa pipo ti endotoxin kokoro arun ti o wa ninu apẹẹrẹ kan.Ohun elo EC64405 ti a ṣe nipasẹ Bioendo ngbanilaaye awọn idanwo 64, ati awọn ipo ifamọ lati 0.1 EU/ml si 1 EU/ml.Bioendo EC64405 ni gbogbo awọn reagents ti o nilo fun idanwo chromogenic ipari-ojuami.Niwọn igba ti a tun ni awọn ohun elo ti ko ni endotoxin tabi awọn ẹya ẹrọ ti ko ni pyrogen, a tun le ṣajọ ohun elo naa da lori ohun ti o nilo.
Incubating Microplate Reader ni a nilo fun idanwo chromogenic ojuami ipari.Oluka microplate incubating wa ELx808IULALXH (awoṣe tuntun labẹ sisẹ ati pe yoo ṣe ifilọlẹ laipẹ) ngbanilaaye lati rii awọn apẹẹrẹ oriṣiriṣi ni 96-daradara microplate ni akoko kanna ati pe yoo ṣe itupalẹ wiwa endotoxin laifọwọyi ati ni deede.
Idanwo idanwo chromogenic LAL ipari-ojuami pẹlu jara meji ti kit, ọkan lo oluka microplate lati ka abajade iṣiro nipasẹ microplate-ọfẹ pyrogen, ekeji lo spectrophotometer deede lati ka abajade titobi nipasẹ awọn tubes gilasi ti ko ni endotoxin.
Lyophilized Amebocyte Lysate / LAL Reagent ti a ṣelọpọ nipasẹ Bioendo jẹ lati inu amebocyte lysate ti o jẹ ẹjẹ ti akan horseshoe.
Katalogi No. | Apejuwe | Awọn akoonu Kit | Ifamọ (EU/ml) |
EC64405 |
Ohun elo Idanwo Bioendo EC Endotoxin (Ayẹwo Chromogenic-ipari), 64 Idanwo / Kit
| 2 Lyophilized Amebocyte Lysate, 1.7ml / vial; 2 Omi fun BET, 50ml / vial; 2 CSE; 4 Chromogenic Sobusitireti, 1.7ml/vial; | 0,1 - 1 EU / milimita |
EC64405S | 0.01 - 0.1 EU / milimita; 0.1 – 1 EU/m |
Lyophilized Amebocyte Lysate / LAL Reagent ti a ṣelọpọ nipasẹ Bioendo jẹ lati inu amebocyte lysate ti o jẹ ẹjẹ ti akan horseshoe.
Katalogi No. | Apejuwe | Awọn akoonu Kit | Ifamọ EU/ml |
EC80545 | Bioendo EC Endotoxin Igbeyewo Apo (Opin-ojuami Chromogenic Ayẹwo, Diazo Coupling), 80 Idanwo / Kit | 5 Lyophilized Amebocyte Lysate, 1.7ml / vial; 4 Omi fun BET, 50ml / vial; 5 CSE; 5 Chromogenic Sobusitireti, 1.7ml/vial; 5 Diazo Reagent 1, 10ml/vial; 5 Diazo Reagent 2, 10ml / vial; 5 Diazo Reagent 3, 10ml / vial; | 0,1 - 1 EU / milimita |
EC80545S | 0.01 - 0.1 EU / milimita; 0,1 - 1 EU / milimita |
Akoko ifiweranṣẹ: Oṣu Kẹsan-15-2019